Characterization of a novel RNA virus from Nesidiocoris tenuis related to members of the genus Iflavirus

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 163, Issue 2
Call Number
ISBN/ISSN 14328798
Author(s) Dong, Yonghao...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Collation
Abstract/Notes Abstract The complete genome of a novel virus from
Nesidiocoris tenuis was determined by RNA-seq and rapid
amplification of cDNA ends. This virus has a single-stranded
RNA genome of 10633 nucleotides (nt) in length, not including
the poly(A) tail, and contains two putative open reading
frames (ORFs). ORF1 encodes a polypeptide of 1320 amino
acids (aa) with a predicted molecular mass of 147.92 kDa
and theoretical isoelectric point (pI) of 6.96. ORF2 encodes
a polypeptide of 1728 aa with a predicted molecular mass
of 197.09 kDa and pI of 6.73. Phylogenetic analysis with
the deduced aa sequences of the conserved RNA dependent
RNA polymerase domain as well as whole genome nt
sequences indicated that the virus clusters with viruses
classified within the genus Iflavirus, with a high bootstrap
value in the maximum-likelihood and neighbor-joining trees.
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