Complete genome sequence of a novel bromovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 163, Issue 2
Call Number
ISBN/ISSN 14328798
Author(s) Safarova, Dana
Candresse, Thierry
Navratil, Milan
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Abstract/Notes Abstract The genus Bromovirus currently contains six
species whose members have relatively narrow host ranges.
In the present work, a new bromovirus infecting elderberry
(Sambucus nigra L.) is reported. dsRNA was purified and
sequenced by next-generation sequencing, and with minimal
additional completion by Sanger sequencing, the full tripartite
genome was obtained. RNA1 is 3241 nt long and contains
ORF1 (1a protein), RNA2 is 2810 nt long and contains
ORF2 (2a protein), and RNA3 is 2244 nt long and contains
ORF3a (movement protein) and ORF3b (coat protein, CP),
separated by an intercistronic poly(A) stretch. Proteins 1a
and 2a showed highest sequence identity (69.9% and 69.4%)
to the corresponding proteins of melandrium yellow fleck
virus. The coat protein showed highest sequence identity
(67.9%) to that of brome mosaic virus. The genome shows
a typical bromovirus organisation comprising of all the
conserved protein domains within the genus. Phylogenetic
analysis supports the assignment of this virus as a new member
of the genus Bromovirus, for which the name “sambucus
virus S” (SVS) is proposed.
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