Serological reactivity to Anaplasma phagocytophilum in neoehrlichiosis patients

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 37, Issue. 9
Call Number
ISBN/ISSN 1435-4373
Author(s) Wass, Linda...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Collation
Abstract/Notes Abstract
The tick-borne bacterium Candidatus (Ca.) Neoehrlichia (N.) mikurensis is a cause of Bfever of unknown origin^ because this
strict intracellular pathogen escapes detection by routine blood cultures. Case reports suggest that neoehrlichiosis patients may
display serological reactivity to Anaplasma (A.) phagocytophilum. Since Anaplasma serology is part of the diagnostic work-up of
undetermined fever in European tick-exposed patients, we wanted to investigate (1) the prevalence of A. phagocytophilum
seropositivity among neoehrlichiosis patients, (2) the frequency of misdiagnosed neoehrlichiosis patients among A.
phagocytophilum seropositive patients, and (3) the frequency of A. phagocytophilum and Ca. N. mikurensis co-infections.
Neoehrlichiosis patients (n = 18) were analyzed for A. phagocytophilum IgM and IgG serum antibodies by indirect immunofluorescence
assay. Serum samples from suspected anaplasmosis patients (n = 101) were analyzed for bacterial DNA contents by
singleplex PCR specific for A. phagocytophilum and Ca. N. mikurensis, respectively. One fifth of the neoehrlichiosis patients (4/
18) were seropositive for IgM and/or IgG to A. phagocytophilum at the time of diagnosis. Among the patients with suspected
anaplasmosis, 2% (2/101) were positive for Ca. N. mikurensis by PCR whereas none (0/101) had detectable A. phagocytophilum
DNA in the serum. To conclude, patients with suspected anaplasmosis may in fact have neoehrlichiosis.We found no evidence of
A. phagocytophilum and Ca. N. mikurensis co-infections in humans with suspected anaplasmosis or confirmed neoehrlichiosis.
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