Early detection of M. leprae by qPCR in untreated patients and their contacts: results for nasal swab and palate mucosa scraping

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 37, Issue. 10
Call Number
ISBN/ISSN 1435-4373
Author(s) Carvalho, Ricardo Sandri...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Abstract/Notes Abstract
To verify if the hard palate mucosa can be a site of relevance in the early molecular detection of Mycobacterium leprae in leprosy
cases and their household contacts and if there is a correlation of results in nasal swab with those of the scraping of the palate
mucosa. The quantitative polymerase chain reaction technique was used. Sample included 78 patients with untreated leprosy
(G1), their 54 household contacts (G2), and 80 healthy individuals for the negative control (G3). The presence of M. leprae in
both G1 and G2 was observed with the nasal swab and the palate mucosa scrapings methods, and it was shown that the sensitivity
between the qPCR exams for RLEP and 85B genes is equivalent, with no statistically significant differences (G1 positivity of
35% in the hard palate mucosa and 44% for the nasal one, p = 0.3731 and for G2 of 31 and 38%, respectively, p = 0.6774).
Results support the fact that the buccal mucosa and nasal mucosa may be important sites of primary infection of leprosy with
repercussion in the transmission chain and that asymptomatic household contacts are heavily harbored by the causative agent of
leprosy, which has a critical significance in the prevention and control action of this disease, since the evaluation of these sites
arises as of importance in the early detection of M. leprae. Close monitoring and chemoprophylaxis of household contacts appear
to be critical to attain interruption of the transmission of leprosy in endemic countries.
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