Intestinal microbiome as a risk factor for urinary tract infections in children

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 37, Issue. 10
Call Number
ISBN/ISSN 1435-4373
Author(s) Paalanne, Niko...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Abstract/Notes Abstract
As urinary tract infection (UTI) pathogens originate from the gut,we hypothesized that the gut environment reflected by intestinal
microbiome influences the risk of UTI. Our prospective case-control study compared the intestinal microbiomes of 37 children
with a febrile UTI with those of 69 healthy children. We sequenced the regions of the bacterial 16S rRNA gene and used the
LefSe algorithm to calculate the size of the linear discriminant analysis (LDA) effect. We measured fecal lactoferrin and iron
concentrations and quantitative PCR for Escherichia coli. At the phylum level, there were no significant differences. At the genus
level, Enterobacter was more abundant in UTI patients with an LDA score > 3 (log 10), while Peptostreptococcaceae were more
abundant in healthy subjects with an LDA score > 3 (log 10). In total, 20 OTUs with significantly different abundances were
observed. Previous use of antimicrobials did not associate with intestinal microbiome. The relative abundance of E. coli was
1.9% in UTI patients and 0.5% in controls (95% CI of the difference—0.8 to 3.6%). The mean concentration of E.coli in
quantitative PCR was 0.14 ng/μl in the patients and 0.08 ng/μl in the controls (95% CI of the difference—0.04 to 0.16). Fecal
iron and lactoferrin concentrations were similar between the groups. At the family and genus level, we noted several differences
in the intestinal microbiome between children with UTI and healthy children, which may imply that the gut environment is linked
with the risk of UTI in children.
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