Evaluation of the Bruker® MBT Sepsityper IVD module for the identification of polymicrobial blood cultures with MALDI-TOF MS

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 37, Issue. 10
Call Number
ISBN/ISSN 1435-4373
Author(s) Scohy, Anaïs...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Collation
Abstract/Notes Abstract
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) considerably reduces timeframe
required from initial blood culture positivity towards complete bacterial identification. However, rapid identification of
polymicrobial blood cultures remains challenging.We evaluated the performances of the Bruker® MBT Sepsityper IVD module
on MALDI-TOF MS for the direct identification of polymicrobial blood culture bottles. This module has the ability to give a
strong indication that a sample contains a mixture of organisms and to identify two of them. Blood culture bottles considered as
polymicrobial using routine subculture were collected and processed using the Sepsityper kit. MALDI-TOF MS identification
was performed using the MBT Compass IVD software including the Sepsityper module. From 143 polymicrobial blood culture
bottles tested, 34.3% (49/143) were completely identified by the module. Both microorganisms were more easily detected by the
module in samples containing two pathogens than in samples containing two contaminants (36.8% vs 29.4%). Additionally, in
more than half of the samples, the module detected 1 of the different microorganisms contained in the same vial. In these cases,
with a pathogen and contaminant in the same sample, the module detected the pathogen in more than 80%. The Sepsityper
module identified 14 microorganisms which were not recovered by conventional culturemethods. The Bruker®MBT Sepsityper
IVD module contributed to a valuable identification of polymicrobial blood cultures in more than a third of all cases.
Conventional culture methods are still required to complete the results and to carry on susceptibility testing.
Keywords MALDI-TOFMS . Direct identification . Polymicrobial blood culture . Bacteremia
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