Use of Andromas and Bruker MALDI-TOF MS in the identification of Neisseria

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 37, Issue. 12
Call Number
ISBN/ISSN 1435-4373
Author(s) Morel, Florence...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2018
Publishing Place Switzerland
Collation
Abstract/Notes Abstract
Through the past decade, MALDI-TOF MS has been recognized as a fast and robust tool for identification of most bacteria in
clinical microbiology. However, the accuracy of this method to identify Neisseria species is still debated, and few data are
available about commensal Neisseria species identification. In this study, we assessed two MALDI-TOF MS systems (Bruker
Biotyper and Andromas) for the identification of 88, 18, and 29 isolates of Neisseria gonorrhoeae, Neisseria meningitidis, and
commensal Neisseria species, respectively. All 88 isolates of N. gonorrhoeae were correctly identified using both systems, and
most N. meningitidis and commensal Neisseria species were well identified: only 1/18 isolates of N. meningitidis was
misidentified using Bruker Biotyper, and 1 isolate of Neisseria polysaccharea was misidentified as N. meningitidis using both
systems. These results strengthen the possibility to use MALDI-TOF MS as a single method for Neisseria identification in
routine, with excellent performance for N. gonorrhoeae identification. However, results should be interpreted prudently for
N. meningitdis and commensal Neisseria species when isolated from genital and oropharyngeal samples where these both species
can coexist.
Keywords Bacterial identification . Neisseria meningitidis . Neisseria gonorrhoeae . Commensal Neisseria species .
Matrix-assisted laser desorption ionization-time of flight mass spectrometry . Bruker Biotyper MALDI-TOF . Andromas
MALDI-TOF
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