Activity of anti-CR3-RP polyclonal antibody against biofilms formed by Candida auris, a multidrug-resistant emerging fungal pathogen

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 38, Issue 1
Call Number
ISBN/ISSN 1435-4373
Author(s) Dekkerova, Jaroslava
Lopez-Ribot, Jose L.
Bujdakova, Helena
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2019
Publishing Place Switzerland
Collation
Abstract/Notes Abstract
Fungal biofilm has remained a serious medical problem that complicates treatment of mycoses. In particular, once biofilms are
formed, they display high levels of resistance against most common antifungals. Candida auris is currently considered as a
serious emerging fungal pathogen frequently exhibiting high levels of resistance to antifungals. Recent studies have confirmed
that C. auris shares similarity with Candida albicans in regards to virulence-associated proteins involved in adherence and
biofilm development. Complement receptor 3-related protein (CR3-RP) is one of the key surface antigens expressed by Candida
species during biofilmformation. Here, we have investigated the presence of this cell surface moiety on the surface of C. auris, as
well as the potential of anti-CR3-RP polyclonal antibody (Ab) to inhibit biofilm formation by this emerging fungal pathogen.
Using indirect immunofluorescence and ELISA, we were able to confirm the presence of CR3-RP in C. auris cells within
biofilms. Further, not only anti-CR3-RPAb was able to inhibit biofilm formation by multiple C. auris strains when added during
the adherence phase, but it also demonstrated activity against C. auris 24-h pre-formed biofilms, which compared favorably to
levels of inhibition achieved by treatment with current conventional antifungals fluconazole, amphotericin B, and caspofungin.
Overall, our data demonstrate the presence of this antigen on the surface of C. auris and points to the potential of anti-CR3-RPAb
in eradication of biofilms formed by this novel fungal pathogen.
Keywords CR3-RP . Polyclonal antibody . Biofilm . Resistance . Candida auris
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