Invasive pulmonary aspergillosis: current diagnostic methodologies and a new molecular approach

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition
Call Number
ISBN/ISSN
Author(s) Moura, S.
Cerqueira, L.
Almeida, A.
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Book
Language English
Publisher Springer Nature Switzerland AG
Publishing Year 2018
Publishing Place Switzerland
Collation
Abstract/Notes Abstract
The fungus Aspergillus fumigatus is the main pathogenic agent responsible for invasive pulmonary aspergillosis.
Immunocompromised patients are more likely to develop this pathology due to a decrease in the immune system’s defense
capacity. Despite of the low occurrence of invasive pulmonary aspergillosis, this pathology presents high rates of mortality,
mostly due to late and unspecific diagnosis. Currently, the diagnostic methods used to detect this fungal infection are conventional
mycological examination (direct microscopic examination, histological examination, and culture), imaging, non-culturebased
tests for the detection of galactomannan, β(1,3)-glucan and an extracellular glycoprotein, and molecular tests based on
PCR. However, most of these methods do not detect the species A. fumigatus; they only allow the identification of genus
Aspergillus. The development of more specific detection methods is of extreme importance. Fluorescent in situ hybridizationbased
molecular methods can be a good alternative to achieve this purpose. In this review, it is intended to point out that most of
the methods used for the diagnosis of invasive pulmonary aspergillosis do not allow to detect the fungus at the species level and
that fluorescence in situ hybridization-based molecular method will be a promising approach in the A. fumigatus detection.
Keywords Invasive pulmonary . Aspergillosis . Diagnostic methodologies . Fluorescence in situ hybridization
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