Comparison between Vitek MS, Bruker Biotyper, Vitek2, and API20E for differentiation of species of the genus Raoultell

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 38, Issue 3
Call Number
ISBN/ISSN 1435-4373
Author(s) Ruiz, Carlos...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2019
Publishing Place Switzerland
Abstract/Notes Abstract
Rapid and reliable identification of microorganisms in the clinical laboratory is essential for an early and accurate diagnosis
guiding timely therapy. However, conventional methods are sometimes unreliable and show controversial outcomes. Matrixassisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a rapid and reliable
method for identification of bacteria and fungi isolated from clinical samples. Members of the genus Raoultella are increasingly
recognized as clinically relevant. There are difficulties in their identification at the species level since sequencing the 16S rRNA
or the rpoB genes does not show conclusive results. The aim of this study has been to compare two MALDI-TOF MS systems
(Vitek MS and Bruker Biotyper) with Vitek2 and API20E systems for differentiation of Raoultella species. A collection of 97
clinical isolates of Raoultella species was identified with Vitek MS, in parallel with Vitek2 and API, and finally with Bruker
Biotyper.Among the twomost widely usedMALDI-TOFMSplatforms, results obtained withVitekMSwere slightly superior to
those obtained with the Bruker Biotyper system, with sensitivities and specificities of 98.9/57.9% and 98.8/37.0%, respectively.
The current commercial phenotypic identification systems are not optimized for the identification of Raoultella species. Our
results indicate that MALDI-TOF-based identification is more accurate and sensitive than that provided by phenotypic methods.
Keywords MALDI-TOFMS . Raoultella . VitekMS . Bruker Biotyper
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