Fourier transform infrared spectroscopy: unlocking fundamentals and prospects for bacterial strain typing

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 38, Issue 3
Call Number
ISBN/ISSN 1435-4373
Author(s) Novais, Angela...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2019
Publishing Place Switzerland
Abstract/Notes Abstract
The need to identify highly related bacterial strains is ancient in clinical, industrial, or environmental microbiology. Strategies
based on different phenotypic and genotypic principles have been used since the early 1930s with variable outcomes and
performances, accompanying the evolution of bacterial features’ knowledge as well as technologies, instruments, and data
analysis tools. Today, more than ever, the implementation of bacterial typing methods that combine a high reliability and accuracy
with a rapid, low-cost, and user-friendly performance is highly desirable, especially for clinical microbiology. FT-IR developments
for bacterial discrimination at the infra-species level settled on the identification of bacterial groups previously defined by
phenotypic or genotypic typing methods. Therefore, this review provides a brief historical overview of main bacterial strain
typingmethods, and a comprehensive analysis of the fundamentals and applications of Fourier transform infrared spectroscopy, a
phenotypic-based method with potential for routine strain typing. The different studies on FT-IR-based strain typing of diverse
Gram-negative and Gram-positive bacterial species are discussed in light of genotypic, phenotypic, and biochemical aspects, in
order to definitively give this methodology credit to be widely accepted by microbiologists. Importantly, the discriminatory
biochemical fingerprints observed on FT-IR spectra have been consistently correlated with sugar-based coating structures that
besides reflecting strain variation are also of high relevance for the specificity in pathogen-host interactions. Thus, FT-IR-based
bacterial typing might not only be useful for quick and reliable strain typing but also to help understanding the diversity,
evolution, and host adaptation factors of key bacterial pathogens or subpopulations.
Keywords Bacterial typing . Strain differentiation . FT-IR spectroscopy . Capsular types . Serotypes . Serogroups . Surface
polysaccharides . Multivariate data analysis
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