O-antigen serotyping and MALDI-TOF, potentially useful tools for optimizing semi-empiric antipseudomonal treatments through the early detection of high-risk clones

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 38, Issue 3
Call Number
ISBN/ISSN 1435-4373
Author(s) Mulet, Xavier...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2019
Publishing Place Switzerland
Abstract/Notes Abstract
The increasing prevalence of extensively drug-resistant (XDR) Pseudomonas aeruginosa infections is due to the
global spread of defined high-risk clones (HRC). Among them, ST175 is particularly frequent in Spain and France.
Here, we evaluated O-antigen serotyping and MALDI-TOF as typing methods for the early identification of ST175.
O-antigen (O4) serotyping and MALDI-TOF biomarker peak-based recognition models were tested in several strain
collections, including 206 non-duplicated P. aeruginosa clinical isolates collected in 2016. Resistance profiles were
determined by broth microdilution and clonal epidemiology by pulsed-field gel electrophoresis (PFGE) and
multilocus sequence typing (MLST). Up to 24.3% of the isolates were XDR and 28.2% non-susceptible to
meropenem, while resistance to ceftolozane/tazobactam (2.9%) and colistin (0.5%) was infrequent. Half of all
XDR isolates belonged to ST175 and most of them were only susceptible to ceftolozane/tazobactam and colistin.
A model based on the detection of one MALDI-TOF biomarker peak yielded negative and positive predicted values
(NPV/PPV) for the detection of ST175 of 100%/51.9%, whereas NPV/PPV for a model based on two biomarker
peaks were 99.4%/87.1% and for O4 serotyping, 99.4%/84.1%. Both, O4 serotyping and MALDI-TOF biomarker
peak analysis, proved to be sensitive and specific methods that could be easily incorporated in the routine workflow
for the early detection of ST175 HCR. Since ST175 is associated with defined XDR profiles, with most isolates only
being susceptible to colistin and ceftolozane/tazobactam, these simple techniques could be useful for optimizing
semi-empiric antipseudomonal treatments in areas where this HRC is prevalent.
Keywords Pseudomonas aeruginosa . High-risk clones . Antibiotic resistance . Ceftolozane-tazobactam . ST175
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