Evaluation of a stepwise approach using microbiota analysis, species-specific qPCRs and culture for the diagnosis of lower respiratory tract infections

Collection Location Koleksi E-book & E-Journal Perpustakaan Pusat Unila
Edition Vol. 38, Issue 4
Call Number
ISBN/ISSN 1435-4373
Author(s) den Munckhof, Ellen H. A. van...[et al.]
Subject(s) Biomedicine
Classification NONE
Series Title
GMD E-Journal
Language English
Publisher Springer
Publishing Year 2019
Publishing Place Switzerland
Collation
Abstract/Notes Abstract
In clinical practice, the diagnosis of lower respiratory tract infections (LRTIs) is based on culture. The aim of this study was to
evaluate whether a stepwise approach using microbiota analysis, species-specific quantitative real-time (q)PCRs and culture has
the potential to be a more accurate and efficient diagnostic approach than culture alone. Sixty-two sputa obtained in a routine
clinical setting from patients with a suspected LRTI were included. All sputa were analysed by culture, microbiota analysis based
on the 16S ribosomal RNA gene and multiple species-specific qPCRs. Microbiota and culture data were compared to investigate
whether cut-off values for microbiota analysis could be determined. For microbiota analysis, a relative abundance of 25% was
identified as the cut-off value for the detection of both genera Streptococcus and Haemophilus. Microbiota analysis combined
with species-specific qPCRs resulted in a significant increase in the number of positive sputa (73% vs 58%; p =0.003) as well as
in the number of identified pathogens (51 vs 37; p = 0.049) compared to culture. A stepwise approach using microbiota analysis,
species-specific qPCRs and culture has the potential to be used in clinical settings for the diagnosis of LRTIs in the near future.
Keywords Culture . Diagnosis . Lower respiratory tract infection . Microbiota analysis . Quantitative real-time PCR . Sputum
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